huvec atcc crl 1730 human  (ATCC)

Journal: Cell Reports Medicine

Article Title: Restoring immune homeostasis in atherosclerotic plaques via inorganic violet phosphorus nano-immunotherapy

doi: 10.1016/j.xcrm.2025.102528

Figure Lengend Snippet: Preparation, characterization, ROS scavenging, and biocompatibility of VPNS@P (A) Schematic of exfoliation and PEGylation of violet phosphorus (VP) to prepare VPNS@P from bulk VP. (B) Transmission electron microscopy (TEM) image of VPNS@P. Scale bar, 100 nm. (C) Hydrodynamic size distribution of VPNS@P measured by dynamic light scattering (DLS). (D and E) Atomic force microscopy (AFM) image (D) and thickness profile (E) of VPNS@P. Scale bar, 100 nm. (F) Raman scattering spectra of VPNS@P. (G) Time-course DLS measurements of VPNS@P incubated in PBS or DMEM supplemented with 10% fetal bovine serum (FBS) over 7 days ( n = 3 independent samples). (H–J) Scavenging capability of VPNS@P ( n = 5 independent samples) toward H 2 O 2 (H), ·OH (I), and O 2 ·− (J). (K–M) Biocompatibility of VPNS@P in vitro . Cell viabilities of RAW264.7 (K), mouse aortic vascular smooth muscle cells (MOVASs) (L), and human umbilical vein endothelial cells (HUVECs) (M) were examined with a CCK-8 assay ( n = 3 biologically independent samples). Data were analyzed using one-way ANOVA with a Dunnett’s T3 post hoc test and are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. ns, not significant.

Article Snippet: Human: HUVEC umbilical vein endothelial cells , ATCC , CRL-1730; RRID: CVCL_2959.

Techniques: Transmission Assay, Electron Microscopy, Microscopy, Incubation, In Vitro, CCK-8 Assay

Journal: Toxicology Research

Article Title: Synthesis, in-vitro, in-silico , and global DNA methylation studies of curcumin-benzoquinone analog in triple-negative breast cancer (TNBC) cells

doi: 10.1093/toxres/tfaf128

Figure Lengend Snippet: The cytotoxicity of JWB1 (3) on HUVEC, MCF-7, and MDA-MB-231 cell lines at a. 24 h B. 48 h C. 72 h. Significant differences compared to controls in MDA-MB-231 = * . Significant differences compared to controls in MCF-7 = +. Significant differences compared between HUVEC and MDA-MB-231 cell lines = #. Significant differences compared between HUVEC and MCF-7 cell lines = ψ * , +, #, ψ = P < 0.05 - * * , ++, # #, ψ ψ = P < 0.01- * * * , +++, ###, ψ ψ ψ = P < 0.001.

Article Snippet: MDA-MB231 (ATCC HTB-26), MCF-7 (ATCC HTB-22), and HUVEC (ATCC-CRL-1730) were cultured in DMEM/F-12 (Thermo Fisher Scientific, USA) medium enriched with fetal bovine serum (10%, FBS, Sigma, USA) and 1% penicillin/streptomycin (Sigma, USA) at 37 °C with 5% CO 2 under standard cell culture conditions.

Techniques:

Journal: Toxicology Research

Article Title: Synthesis, in-vitro, in-silico , and global DNA methylation studies of curcumin-benzoquinone analog in triple-negative breast cancer (TNBC) cells

doi: 10.1093/toxres/tfaf128

Figure Lengend Snippet: Global DNA methylation levels of HUVEC, MDA-MB-231, and MCF-7 cell lines. Significant differences compared to controls in MDA-MB-231 = * Significant differences compared to controls in MCF-7 = + Significant differences compared between HUVEC and MDA-MB-231 cell lines = #Significant differences compared between HUVEC and MCF-7 cell lines = ψ * , + , # , ψ = P < 0.05 - * * , ++ , # # , ψ ψ = P < 0.01- * * * , +++ , ### , ψ ψ ψ = P < 0.001.

Article Snippet: MDA-MB231 (ATCC HTB-26), MCF-7 (ATCC HTB-22), and HUVEC (ATCC-CRL-1730) were cultured in DMEM/F-12 (Thermo Fisher Scientific, USA) medium enriched with fetal bovine serum (10%, FBS, Sigma, USA) and 1% penicillin/streptomycin (Sigma, USA) at 37 °C with 5% CO 2 under standard cell culture conditions.

Techniques: DNA Methylation Assay